BW766 

TCBS AGAR / (SELECTIVE)

Formula

Ingredients 

gms/lit.

Peptone, special

Yeast extract

Sodium thiosulphate

Sodium citrate

Sodium cholate

Oxgall

Sucrose

Sodium chloride

Ferric citrate

Bromothymol blue

Thymol blue

Agar

10.00

5.00

10.00

10.00

3.00

5.00

20.00

10.00

1.00

0.04

0.04

15.00

Final pH (at 25°C) : 8.6 + 0.2

Directions 

Suspend 89 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C and pour into petri plates.

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