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BW015 |
BRILLIANT GREEN BILE BROTH 2% |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Lactose Oxgall Brilliant green |
10.00 10.00 20.00 0.0133 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 40 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Distribute in fermentation tubes containing inverted Durham’s tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Do not autoclave double strength broth. NOTE: Where number of organisms is expected to be low, large quantities of samples may be added with equal quantities of double strength medium in dilution bottles. |
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