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BS033 |
NIACIN ASSAY MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Casein acid hydrolysate vitamin free Dextrose Sodium acetate L-Cystine DL- Tryptophan Adenine sulphate Guanine hydrochloride Uracil Thiamine hydrochloride Calcium pantothenate Pyridoxine hydrochloride Riboflavin p-Amino benzoic acid Biotin Dipotassium phosphate Monopotassium phosphate Magnesium sulphate Sodium chloride Ferrous sulphate Manganese sulphate |
12.00 40.00 20.00 0.40 0.20 0.02 0.02 0.02 0.0002 0.0002 0.0004 0.0004 0.0001 0.00008 1.00 1.00 0.40 0.02 0.02 0.02 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 7.51 gms. in 100ml distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. For the assay, dispense 5 ml medium per assay tube (containing increasing amounts of standard or unknown) and make up the total volume to 10ml per tube with distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. Cool immediately. Generally satisfactory results are obtained with Niacin or Niacinamide at levels of 0, 0.01, 0.02, 0.04, 0.06, 0.08, 0.1, 0.12 micrograms per assay tube (10ml). |
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