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BS020 |
B 12 CULTURE AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Tryptone Yeast extract Sucrose Sodium chloride Di-potassium phosphate Magnesium sulphate Liver extract Ferrous sulphate Agar |
5.00 5.00 12.00 5.00 0.50 0.20 0.05 0.001 10.00 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 32.8 gms. in 1000ml. Distilled water. Boil to dissolve the medium completely. Dispense in 10 ml amounts in tubes and sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. |
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