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BS016 |
VITAMIN B12 ASSAY MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Casein acid hydrolysate Dextrose Aspargine Sodium acetate Ascorbic acid L-cystine DL-tryptophan Adenine sulphate Guanine hydrochloride Uracil Xanthine Riboflavin (vitamin B2) Thiamine hydrochloride Biotin Niacin p-Amino benzoic acid Calcium pantothenate Pyridoxine hydrochloride Pyridoxal hydrochloride Pyridoxamine hydrochloride Folic acid Monopotassium phosphate Dipotassium phosphate Magnesium sulphate Sodium chloride Ferrous sulphate Manganese sulphate Polysorbate |
15.00 40.00 0.20 20.00 4.00 0.40 0.40 0.02 0.02 0.02 0.02 0.001 0.001 0.00001 0.002 0.002 0.001 0.004 0.004 0.0008 0.0002 1.00 1.00 0.40 0.02 0.02 0.02 80 2.00 |
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Final pH (at 25°C) : 6.1 + 0.2 |
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Directions |
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Suspend 8.5 gms. in 100ml. distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. For the assay, dispense 5 ml. medium to each assay tube (containing increasing amounts of standard of the unknown).Total volume of 10 ml per tube is adjusted by addition of distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 5 minutes. Cool the medium immediately. Generally satisfactory results are obtained with Vitamin B12 (cyanocobalamin) at levels 0, 0.025,0.05,0.075,0.1,0.125,0.15,0.2 ng per assay tube (10ml.) |
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