|
BF012 |
BRUCELLA SELECTIVE SUPPLEMENT,MODIFIED |
|
|
Formula |
||
|
Ingredients |
(per vial sufficient for 500 ml. of medium) |
|
|
Polymixin B Bacitracin Nystatin Natamycin Nalidixic acid Vancomycin |
2500 IU 12500 IU 50000 IU 50.00 mg 2.50 mg 10.00 mg |
|
|
Directions |
||
|
Rehydrate the contents of 1 vial aseptically with 5 ml.of 50% methanol. Shake to form uniform suspension. Add the contents to 500 ml of sterile molten media such as Blood agar base no.2(B116,B117)/Columbia Blood agar base(B994,B148) with 5-10% v/v inactivated horse serum and 1% w/v sterile dextrose or Brucella Agar base(B125)/ Brucella Broth base(B126) with 5-10% v/v inactivated horse serum. Mix well and pour into sterile Petri plates/tubes. |
||