B978

CHARCOAL AGAR W/ NIACIN

Formula 

B978

Ingredients 

gms/lit.

Pancreatic digest of gelatin 

Beef extract 

Sodium chloride 

Starch,soluble 

Charcoal 

Nicotinic acid 

Agar 

10.00

10.00

5.00

 10.00

4.00

0.01

 12.00

Final pH (at 25°C) :  

7.4 + 0.2

Directions

Suspend 51 gms. of B978 in 1000 ml. distilled water. Boil to dissolve the medium with frequent stirring. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add sterile 50 ml of defibrinated blood and Bordetella Selective Supplement . 

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