B965 

CAMPYLOBACTER CEFEX AGAR

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate 

Peptic digest of animal tissue 

Yeast extract 

Sodium chloride 

Glucose 

Ferrous sulphate 

Sodium pyruvate

Sodium bisulphate 

Agar 

15.00

10.00

2.00

5.00

1.00

0.50

0.50

0.35

15.00

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 49.35 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 50°C. Aseptically add 10% v/v defibrinated sheep blood or 5.7% v/v laked horse blood and rehydrated contents of one vial of Park and Sanders selective supplement II. Mix well and pour into sterile petri plates.

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