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B961 |
CRAMP AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein acid hydrolysate Galactose Sodium chloride Morpholine propane sulfonic acid Ammonium chloride Sodium thiosulphate Dipotassium phosphate Magnesium sulphate Tricine Congo red Agarose |
2.00 2.00 2.90 8.40 0.50 0.60 0.24 0.0986 1.80 0.005 14.00 |
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Final pH (at 25°C) : 5.3 + 0.2 |
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Directions |
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Suspend 32.54 gms. in 1000 ml. distilled water. Heat if necessary to dissolve the medium completely. Distribute into tubes or flasks. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. |
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