B960 

C.P.C AGAR BASE

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract 

Cellobiose 

Sodium chloride 

Bromo thymol blue 

Cresol red

Agar 

10.00

5.00

15.00

20.00

0.04

0.04

15.00

Final pH (at 25°C) : 7.6 + 0.2

Directions 

Suspend 32.54 gms. in 500 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add reconstituted contents of 1 vial of CPC supplement. Mix well.

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