B959

C.L.E.D. AGAR WITHOUT INDICATOR

Formula 

B959

Ingredients 

gms/lit.

Peptic digest of animal tissue 

Casein enzymic hydrolysate 

Beef extract 

Lactose 

L-cystine 

Agar 

 4.00

4.00

3.00

10.00

0.128

 15.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend  36.1 gms. of B959 in 998 ml. distilled water. Add rehydrated contents of 1 vial of Bromo Thymol Blue Supplement . Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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