B948 

BREWER THIOGLYCOLLATE MEDIUM MODIFIED

Formula 

B948

Ingredients 

gms/lit.

Casein enzymic hydrolysate 

Papaic digest of soyabean meal 

Dextrose 

Sodium chloride 

Dipotassium phosphate 

Sodium thioglycollate 

Methylene blue 

Agar 

17.00

3.00

10.00

5.00

2.00

1.00

0.002

0.50

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 38.5 gms.  in 1000 ml. distilled water. Boil to dissolve the medium completely. Dispense in tubes or in suitable containers as desired and sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.

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