B939 

BACTERIODES BILE ESCULIN AGAR (BBE)

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate 

Papaic digest of soyabean meal

Sodium chloride

Oxgall 

Esculin 

Ferric ammonium citrate 

Hemin 

Agar 

15.00

5.00

5.00

20.00

1.00

0.50

0.01

15.00

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 61.51 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of Bacteroides Selective Supplement. Mix well and pour into sterile petri plates.

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