B914      

ACID BROTH

Formula 

Ingredients

gms/lit.

Peptic digest of animal tissue

Yeast extract

Invert sugar 

10.00

7.50

10.00

Final pH (at 25°C) : 4.0 + 0.2

Directions 

Suspend 27.5 gms. in 1000 ml. distilled water. Heat if necessary to dissolve the medium completely. Distribute into tubes or flasks. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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