B911  

ACETAMIDE BROTH (TWIN PACK)

Formula

B911

Ingredients

gms/lit.

Part A 

Acetamide 

Part B 

Sodium chlroride   

Dipotassium hydrogen phosphate

Potassium dihydrogen phosphate 

Magnesium sulphate

Phenol red

 

 

10.00

 

5.00

1.39 

0.73 

0.50 

0.012

 

Final pH (at 25°C)

7.0 + 0.2

Directions

Suspend  7.63 gms. of part B in 1000 ml distilled water. Add 10 gms of Part A. Heat to boiling to dissolve the medium completely. Dispense in tubes or as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the agar tubes in slanting position.

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