B900

 A 1 MEDIUM

Formula

Ingredient

grm/lit

Casein enzymic hydrolysate

Lactose

Sodium chloride

Salicin

Polyethlene glycol p-isooctylphenylether (Triton 100)

20.00

5.00

5.00

0.50

1 ml

Final pH (at 25°C) :  6.9 + 0.2 

Directions

Suspend 31.5 gms. in 1000 ml. distilled water. Heat to dissolve the medium completely. Distribute 10 ml.amounts into tubes containing inverted Durham’s tubes. Sterilize by autoclaving at 15 lbs pressure (121° C) for 10 minutes.

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