B862 

M-TETRATHIONATE BROTH BASE

Formula

Ingredients 

gms/lit.

Proteose peptone

Bile salts

Sodium thiosulphate

5.00

1.00

30.00

Final pH (at 25°C) : 8.0 + 0.2

Directions 

Suspend 3.6 gms. in 100ml distilled water. Heat to boiling to dissolve the medium completely. Cool below 45°C and add 2ml iodine solution containing 0.5 grams potassium iodide and 0.6 gms. iodine crystals. Complete medium should be used on the day of preparation.

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