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B850 |
M-BRILLIANT GREEN BROTH |
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Formula |
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Ingredients |
gms/lit. |
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Proteose peptone Yeast extract Lactose Saccharose Sodium chloride Phenol red Brilliant green |
20.00 6.00 20.00 20.00 10.00 0.16 0.25 |
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Final pH (at 25°C) : 6.9 + 0.2. |
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Directions |
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Suspend 76.13 gms. in 1000ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 35°C and saturate sterile absorbent cotton pad with 2ml of the broth. The medium should be used within 24 hours of rehydration |
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