B841

LACTOBACILLUS SELECTION AGAR

Formula 

B841 

Ingredients 

gms/lit.

Casein enzymic hydrolysate 

Yeast extract 

Glucose 

Sodium acetate 

Monopotasium hydrogen phosphate 

Ammonium citrate 

Polysorbate 80 

Magnesium sulphate 

Manganese sulphate 

Ferrous sulphate 

Agar

10.00 

5.00 

20.00 

25.00 

6.00

2.00 

1.00 

0.575 

0.12

0.34

15.00 

Final pH (at 25°C) : 5.5 + 0.2 5.4 ± 0.2

Directions 

Suspend 84.7 gms. of B841 in 1000ml. distilled water containing 1.32ml. glacial acetic acid. Heat with frequent stirring. Boil for 1-2 minutes to dissolve the medium completely. DO NOT AUTO CLAVE. If storage is necessary, autoclave at 12 lbs pressure (118°C) for 15 minutes.

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