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B833 |
SORBITOL IRON AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Beef extract Proteose peptone D-sorbitol Sodium chloride Ferric ammonium citrate Sodium thiosulphate Phenol red Agar |
3.00 15.00 2.00 5.00 0.50 0.50 0.03 20.00 |
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Final pH (at 25°C) : 7.6 + 0.2 |
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Directions |
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Suspend 46 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in test tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubes to cool in slanted position. |
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