B808 

V-8 MEDIUM FOR LACTOBACILLI

Formula

Ingredients 

gms/lit.

V-8 vegetable juice

Tryptose

Beef extract

Lactose

Bromo cresol green

Agar

10.00

10.00

3.00

5.00

0.10

15.00

Final pH (at 25°C) : 5.7 + 0.2

Directions 

Suspend 43.10 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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