B795 

TRYPTONE PHOSPHATE BROTH

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Dipotassium phosphate

Monopotassium phosphate

Sodium chloride

Polysorbate 80

20.00

2.00

2.00

5.00

1.50

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 30.5 gms. in 1000ml. distilled water. Heat if necessary to dissolve the medium completely. Dispense in 100 ml. aliquots and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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