B776 

THIOGLYCOLLATE AGAR

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

L Cystine

Dextrose

Yeast extract

Sodium chloride

Sodium thioglycollate

Rresazurin

Agar

15.00

0.50

5.00

5.00

2.50

0.50

0.001

20.00

Final pH (at 25°C) : 7.1 + 0.2

Directions 

Suspend 48.5 gms. in 1000 ml. distilled water Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for15 minutes.

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