B766 

TCBS AGAR / (SELECTIVE)

Formula 

B766

Ingredients 

gms/lit.

Peptone special

Yeast extract 

Sodium thiosulphate 

Sodium citrate 

Sodium cholate

Oxgall 

Sucrose 

Sodium chloride 

Ferric citrate 

Bromo thymol blue 

Thymol blue 

Agar 

10.00

5.00

10.00

10.00

3.00

5.00

20.00

10.00

1.00

0.04

0.04

15.00

Final pH (at 25°C)

8.8 + 0.2

Directions 

Suspend 89 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C and pour into petri plates.

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