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B762 |
SULPHATE REDUCING MEDIUM (Triple Pack) |
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Formula |
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Ingredients |
gms/lit. |
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Part A Peptic digest of animal tissue Beef extract Magnesium sulphate Sodium sulphate Dipotassium phosphate Calcium chloride Part B Ferrous ammonium sulphate Sodium ascorbate Part C Sodium lactate |
2.00 1.00 2.00 1.50 0.50 0.10
0.392 0.10
3.50 |
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Final pH (at 25°C) : 7.5 + 0.2 |
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Directions |
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Suspend 7.1 gms. of Part A in 900ml. distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. On the day of use prepare solution of Part B in 100 ml. distilled water. Sterilize by filtration through a 0.45 um membrane filter and aseptically add this 100 ml. solution to 900 ml. Part A medium. Then separately sterilize the Part C by autoclaving at 15 lbs pressure (121°C) for 15 minutes and aseptically add to the mixture of Part A & B. Mix well and aseptically transfer the complete medium to sterile screw capped tubes filling them completely. |
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