B749 

STREPTOCOCCUS ENRICHMENT BROTH (SE BROTH)

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Yeast extract

Bovine bile

Sodium chloride

Sodium citrate

Esculin

Ferric ammonium citrate

Sodium azide

20.00

5.00

10.00

5.00

1.00

1.00

0.50

0.25

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 42.8 gms. in 1000ml. distilled water. Heat to dissolve the medium completely. Dispense in 9 ml. amounts into test tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 20 minutes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 20 minutes.

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