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B741 |
STAPHYLOCOCCUS AGAR NO.110 / W/ AZIDE |
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Formula |
B741 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Gelatin Lactose D-Mannitol Sodium chloride Dipotassium phosphate Sodium azide Agar |
10.00 2.50 30.00 2.00 10.00 75.00 5.00 0.10 15.00 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 149.6 gms. of B741 in 1000ml. distilled water. Mix thoroughly. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Resuspend the precipitate by gentle agitation to avoid bubbles and pour the plates while the medium is hot. Alternatively, cool the medium to 45-50°C and add blood or egg yolk if desired. Staphylococcus Agar No.110 may also be used without sterilization; it should be boiled for 5 minutes and used at once. |
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