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B697 |
Mn AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Beef extract Yeast extract Manganous carbonate Ferrous ammonium sulphate Sodium citrate Agar |
1.00 0.075 2.00 0.15 0.15 12.00 |
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Final pH (at 25°C) : Self |
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Directions |
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Suspend 15.4 gms. in 1000ml distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 50-55°C and aseptically add filter sterilized solution of Cyanocobalamin to a final concentration of 0.005 mg/litre. |
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