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B696 |
PURPLE AGAR |
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Formula |
B696 |
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Ingredients |
gms/lit. |
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Peptone, special Beef extract Sodium chloride Bromo cresol purple Agar |
10.00 1.00 5.00 0.02 15.00 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 31 gms. of B696 in 1000ml. distilled water. Add 5-10 gms. of the carbohydrate to be tested. Dispense in tubes as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Alternatively sterilize the basal medium using 900 ml. distilled water and add 100 ml. separately sterilized 5- 10% solution of the desired carbohydrate to it. |
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