B695 

PSEUDOMONAS AGAR BASE

Formula

Ingredients 

gms/lit.

Pancreatic digest of gelatin 

Casein enzymic hydrolysate

Potassium sulphate 

Magnesium chloride. 6H2O 

Agar 

16.00

10.00

10.00

1.40

11.00

Final pH (at 25°C) : 7.1 + 0.2

Directions 

Suspend 24.2 gms. in 500ml. distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C & aseptically add sterile rehydrated contents of either Cetrinix Supplement or CFC supplement as desired. Mix well & pour into sterile petri plates.

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