B692 

MODIFIED BILE ESCULIN AZIDE AGAR

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Peptic digest of animal tissue

Yeast extract

Oxgall

Sodium chloride

Sodium citrate

Esculin

Ferric ammonium citrate

Sodium azide

Agar

17.00

3.00

5.00

10.00

5.00

1.00

1.00

0.50

0.25

13.50

Final pH (at 25°C) : 7.1 + 0.2.

Directions 

Suspend 56.25 gms. in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes

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