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B685 |
PHENYLETHYL BLOOD AGAR BASE (ANAEROBIC) |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Papaic digest of soyabean meal Yeast extract Sodium chloride Phenylethyl alcohol L-cystine Vitamin K1 Hemin Agar |
15.00 5.00 5.00 5.00 2.50 0.40 0.01 0.005 20.00 |
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Final pH (at 25°C) : 7.5 + 0.2 |
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Directions |
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Suspend 52.92 gms. in 950ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45- 50°C. Aseptically add sterile 50 ml. defibrinated sheep or rabbit blood. Mix well and pour into sterile petri plates. |
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