B651 

MODIFIED DIFFERENTIAL CLOSTRIDIAL BROTH

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Meat peptone

Meat extract

Yeast extract

Starch

Dextrose

L-Cysteine hydrochloride

Sodium acetate

Sodium disulphite

Ammonium ferric citrate

Resazurin sodium

5.00

5.00

8.00

1.00

1.00

1.00

0.50

5.00

0.50

0.50

0.002

Final pH (at 25°C) : 7.1 + 0.2

Directions 

Suspend 27.5 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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