B628I

MODIFIED RAPPAPORT VASSILIADIS MEDIUM

Formula 

B628I

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Sodium chloride

Monopotassium phosphate

Magnesium chloride 6H2O

Malachite green

5.00

8.00

1.60

40.00

0.04

 Final pH (at 25°C) : 5.2 + 0.2

Directions 

Suspend 30 grams of dehydrated medium in 1000 ml distilled water. Heat gently if necessary to dissolve the medium completely. Dispense as desired into tubes and sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes.

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