B618 

TRYPTONE SOYA AGAR WITH LECITHIN AND TWEEN 80

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Papaic digest of soyabean meal

Sodium chloride

Lecithin

Polysorbate 80 (Tween 80)

Agar

15.00

5.00

5.00

0.70

5.00

15.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 45.7 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 12 to 15 lbs pressure (118-121°C) for 15 minutes.

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