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B615 |
PIKE STREPTOCOCCAL BROTH |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Tryptose Yeast extract Dextrose Sodium azide Crystal violet |
10.00 10.00 10.00 0.20 0.065 0.002 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 30.3 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in 100 ml. amounts in flasks. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 5% v/v sterile defibrinated rabbit blood. Mix well and dispense aseptically in 2 ml. amounts in sterile tubes. |
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