B610 

MANNITOL MOTILITY TEST MEDIUM

Formula

Ingredients 

gms/lit.

Mannitol

Phenol red 

Peptic digest of animal tissue 

Potassium nitrate 

Agar 

2.00

0.04

20.00

1.00

3.00

Final pH (at 25°C) : 6.8 + 0.2

Directions 

Suspend 26 gms. in 1000ml. distilled water. Heat boiling to dissolve the medium completely. Dispense in test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubed medium in upright position.

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