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B599 |
MIU MEDIUM BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Dextrose Sodium chloride Phenol red Agar |
10.00 1.00 5.00 0.01 2.00 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 18 gms. in 950 ml distilled water. Boil to dissolve the medium Dispense in 95 ml. amounts into flasks & sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C-55°C & aseptically add 5 ml. sterile 40% Urea solution per 95 ml. |
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