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B596 |
LYSINE IRON CYSTINE BROTH BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract L-Lysine hydrochloride Mannitol Dextrose Salicin L-Cystine Ferric ammonium citrate Sodium thiosulphate Neutral red |
5.00 3.00 10.00 5.00 1.00 1.00 0.10 0.50 0.10 0.025 |
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Final pH (at 25°C) : 6.2 + 0.2 |
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Directions |
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Suspend 25.7 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs Pressure (121°C) for 15 minutes. Cool to room temperature and one vial of reconstituted Novobiocin Selective Supplement. Mix well before dispensing in sterile tubes. |
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