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B226/ B591 |
LITTMAN OXGALL BROTH BASE |
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Formula |
B591 |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Diastase Oxgall Crystal violet |
10.00 10.00 15.00 0.01 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 35 gms. of B591 in 1000ml. distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add sterile Streptomycin to a final concentration of 30 mcg/ml of medium. |
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