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B567 |
K.R.A.N.E.P. AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Sodium chloride Yeast extract Beef extract Potassium thiocyanate Sodium pyruvate Mannitol Lithium chloride Sodium azide Cycloheximide Agar |
5.00 5.00 1.50 1.50 25.50 8.20 5.10 5.10 0.05 0.041 15.00 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 72 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50 °C and aseptically add sterile 100 ml of Egg Yolk Emulsion (BF003). Mix well before pouring. |
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