B519

ORANGE SERUM AGAR

Formula

B519 

Ingredients 

gms/lit.

Casein enzymic hydrolysate 

Yeast extract  

Dextrose  

Dipotassium phosphate

Orange serum (solids from 200ml)

Agar  

10.00 

3.00

4.00

2.50

9.00

17.00

Final pH (at 25°C) : 5.5 + 0.2

Directions

Suspend 45.5 gms. of B519 in 1000ml. distilled water. Heat boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING.

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