B516

ORCHID AGAR

Formula

Ingredients

gms/lit.

Calcium nitrate

Monopotassium dihydrogen phosphate

Magnesium sulphate

Ammonium sulphate

Ferrous sulphate

Manganese sulphate

Saccharose

Agar

1.00

0.25

0.25

0.50

0.025

0.0075

20.00

15.00

Final pH (at 25°C) : 5.0 + 0.2

Directions

Suspend 37 gms. in 1000ml distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.

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