B512 

EGG YOLK AGAR , MODIFIED

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Papaic digest of soyabean meal

Yeast extract 

Sodium chloride 

L-Cystine

Hemin 

Vitamin K1 

Agar

15.00

5.00

5.00

5.00

0.40

0.005

0.01

20.00

Final pH (at 25°C) : 7.5 + 0.2

Directions 

Suspend 50.4 gms. in 900 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50-55°C and aseptically add 100ml Egg Yolk Emulsion. Mix well and pour into sterile petri plates.

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