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B511 |
EGG YOLK AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Proteose peptone Disodium phosphate Monopotassium phosphate Sodium chloride Magnesium sulphate Glucose Hemin Agar |
40.00 5.00 1.00 2.00 0.10 2.00 0.005 25.00 |
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Final pH (at 25°C) : 7.6 + 0.2 |
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Directions |
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Suspend 75 gms. in 900ml.distilled water. Heat to boiling to dissolve the medium completely. Dispense in 90 ml amounts and Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min. Cool to 50-55°C. Aseptically add 10ml of sterile egg yolk emulsion per 90 ml of sterile medium. Mix well. |
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