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B483 |
DECARBOXYLASE AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Yeast extract Dextrose Bromo cresol purple Agar |
5.00 3.00 1.00 0.02 15.00 |
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Final pH (at 25°C) : 6.5 + 0.2 |
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Directions |
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Suspend 24 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Add 5 gms. of desired L-Amino acid (L-Lysine, L-Arginine, L-Ornithine) in hydrochloride form per litre of the medium. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Dispense into sterile test tubes and cool in a slanted position .When L-ornithine hydrochloride is used, readjustment of pH is necessary. |
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