|
B476 |
CRYSTAL VIOLET LACTOSE BROTH |
|
|
Formula |
||
|
Ingredients |
gms/lit. |
|
|
Peptic digest of animal tissue Lactose Dipotassium phosphate Monopotassium phosphate Crystal violet |
5.00 5.00 5.00 1.00 0.00143 |
|
|
Final pH (at 25°C) : 7.4 + 0.2 |
||
|
Directions |
||
|
Suspend 16 gms. in 1000 ml. distilled water. Distribute in 10 ml quantities in tubes with inverted Durham tubes. Sterilize by autoclaving at 15lbs pressure for 15 minutes. If the inoculum is more than 1 ml. double or multiple strength media should be used. |
||