B472 

PL AGAR

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Yeast extract

Sodium chloride

Mannitol

L-Arabinose

Inositol

L-Lysine

Bile salts

Phenol red

Agar

5.00

2.00

5.00

7.50

5.00

1.00

2.00

1.00

0.08

15.00

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 43.58 gms. in 1000ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 10lbs pressure (115°C) for 15 minutes. 

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