B450 

CANDIDA BCG AGAR BASE

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Yeast extract

Dextrose

Bromo cresol green

Agar

10.00

1.00

40.00

0.02

15.00

Final pH (at 25°C) :6.1 + 0.2

Directions 

Suspend 66 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and add sterile Neomycin to a concentration of 500 mcg/ml. of medium. Mix well before pouring into sterile petri plates.

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