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B447 |
C. PERFRINGENS SPORULATION BROTH |
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Formula |
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Ingredients |
gms/lit. |
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Tryptose Yeast extract Starch,soluble Magnesium sulphate Sodium thioglycollate Disodium phosphate |
15.00 3.00 3.00 0.10 1.00 11.00 |
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Final pH (at 25°C) : 7.8 + 0.2. |
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Directions |
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Suspend 33.1 gms. in 1000 ml. distilled water. Heat if necessary to ensure complete solution. Dispense 20 ml amounts in 20 X 150 mm screw capped test tubes. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Just before use heat the medium in flowing steam for 20 minutes |
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